2018 - 2023

Innovations in antimicrobial alternatives and stewardship in poultry production

Principal Investigator: Shayan Sharif, University of Guelph
Co-investigator: Doug Korver, University of Alberta; John Parkinson, Hospital for Sick Children/University of Toronto; Juan Carlos Rodriguez-Lecompte, University of PEI; Joshua Gong, Agriculture and Agri-Food Canada
Status: In progress


The overall Objective is to design, construct and develop a feasible and effective necrotic enteritis (NE)-vaccine strategy in chickens with specific objectives as below.
  1. Development of C. perfringens culture supernatant-based oral vaccine against NE
  2. Design and development of a probiotic-adjuvanted oral vaccine against NE
  3. Construction and development of a recombinant probiotic vectored vaccine expressing C. perfringens protective proteins for prevention of NE.
  4. Farm level evaluation of safety and efficacy profiles of vaccine formulations


Dietary antibiotics have been widely used in poultry production to promote bird growth and performance and prevent economically important diseases such as NE. The spread of antibiotic resistance threatens human health and efforts to restrict dietary antibiotics in poultry production in Canada are rapidly underway. Importantly, the Canadian poultry industry has identified NE as one of the most important diseases. Therefore, identifying safe and efficacious alternatives to antibiotics leading to NE control is urgently needed. Vaccination offers an effective alternative, however, there is currently no effective vaccine available for NE control in poultry. Therefore, the proposed research is anticipated to yield a novel vaccine-based NE prevention strategy to replace the widespread global use of prophylactic antibiotics in chickens raised in the present-day intensive rearing systems.


$1,868,883 (AAFC/CPRC $1,330,063*, OMAFRA $202,000, George Weston Seeding Food Innovation $150,000, Alberta Agriculture Funding Consortium $101,820, Lallemand Inc $45,000, Compute Canada $40,000 (in-kind))

*This research is part of the 2018-2023 Poultry Science Cluster which is supported by AAFC as part of the Canadian Agricultural Partnership, a federal-provincial-territorial initiative.

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