2010 - 2013

Development of Salmonella Enteritidis vaccine for layers and breeding hens

Principal Investigator: Martine Boulianne, University of Montreal
Status: Completed


  • To develop a new vaccine against Salmonella Enteriditis for laying and breeder hens.
  • To verify post-vaccination immune response and efficacy of the vaccine in challenged chicks


Infection by Salmonella spp. is a major cause of human foodborne illness, with Salmonella Enteritidis (SE) being amongst the most prevalent type. SE outbreaks are typically associated with egg consumption, but in recent years, the bacteria are increasingly being found in broiler chickens. Vaccination has been a suggested strategy to prevent SE contamination in laying hens and several killed (inactivated) vaccines are available to Canadian egg producers; however, Dr. Boulianne’s team previously showed studies that, while these vaccines did elicit immune responses throughout the production period, they were not effective in preventing SE-challenged hens from shedding the bacteria or laying positive SE eggs. The researchers hypothesize that creating a subunit vaccine (which uses a protein to initiate an immune response) along with a new delivery method involving encapsulation will effectively control SE infection in hens and chicks.


Several candidate proteins have been selected that could be used in the vaccine and the researchers are currently evaluating the immune responses to each.


Development of a new vaccine that effectively reduces SE infection in the hen, reduces shedding and protects the chick would have positive food safety implications.


$164,165 AAFC


This research was part of the 2010-2013 Poultry Science Cluster which was supported by AAFC as part of Growing Forward, a federal-provincial-territorial initiative.c

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